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#Grand Rounds

A Better Test for Zika?

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PURPOSE:

This study by Wong et al. (EBioMedicine, 2017) aimed to find a rapid and more accurate test for the Zika virus (ZIKV) using multiplex microsphere immunoassay (MIA).

METHODS:

Laboratory Validation Study

RESULTS:

153 patient specimens with ZIKV and/or dengue virus (DENV) were tested. This study found that ZIKV enveloped-based MIA is at least as sensitive as IgM-capture ELISA (the standard diagnostic tool) in diagnosing ZIKV. The antibody response to the viral nonstructural proteins NS1 and NS5 is more specific to ZIKV than the antibody response to envelope protein alone. Including these proteins in the MIA increases the diagnostic accuracy. This diagnosis is also rapid, with a turnaround time of less than four hours, and requires only 10μl for a single reaction.

Learn More – Primary Sources:

A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis

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Related ObG Topics:

CDC Guidance for Non-Pregnant Patients and Available Diagnostic Tests for Zika Virus
Zika Virus Disease: How to Counsel Your Patient About Prevention
Zika virus: transmission, symptoms and management

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